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. 2008 Dec 2;18(2):268–276. doi: 10.1002/pro.29

Table I.

Unfolding Kinetic Parameters of MBP, MBP•Maltose Complex, and RNase H Determined by Pulse Proteolysis and Circular Dichroism in Urea

Protein Method ku(H2O) (s−1) mu (kcal/(mol M))
MBP Pulse proteolysis (6.0 ± 1.6) × 10−7 0.96 ± 0.04
CD (12.4 ± 1.1) × 10−7 0.87 ± 0.01
MBP + 100 μM maltose Pulse proteolysis (9.4 ± 3.2) × 10−9 1.01 ± 0.03
RNase H Pulse proteolysis (2.1 ± 0.1) × 10−5 0.40 ± 0.03
CDa (1.69 ± 0.04) × 10−5 0.42 ± 0.03
a

From Ref.11.