Table I.
96-Well Plate Assays of Aβ42 Solubility
| Reporter construct | FI/ODa | SDb | CV (%)c | S-to-Bd | SD above meane |
|---|---|---|---|---|---|
| ssTorA-Aβ42-Bla | 1111 | 55 | 5.0 | 1.0 | — |
| ssTorA-GM6-Bla | 2566 | 154 | 6.0 | 2.3 | 9.4 |
| Aβ42-Bla | 1144 | 62 | 5.4 | 1.0 | 0.5 |
| GM6-Bla | 1198 | 67 | 5.6 | 1.1 | 1.3 |
Specific fluorescence activity calculated as the fluorescence intensity (FI) divided by the optical density (OD) (average of 96 readings).
Standard deviation (SD) from measurements across an entire plate.
Coefficient of variance (CV) calculated as the (SD/FI/OD)*100 across an entire plate.
Signal-to-background (S-to-B) calculated by normalizing signals to the FI/OD for ssTorA-Aβ42-Bla negative control.
Number of standard deviations above the average of the ssTorA-Aβ42-Bla negative control.