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15N HSQC assignments for mPrP(113–231). (a). Mouse PrP sequence of the C-terminal domain used in this study. (b) Annotated 15N HSQC spectrum. Numbering relates to the sequence in (a). The C-terminal His-tag (232–239) is unassigned. Dashed lines connect side chains of unassigned Q and N sidechains. Boxes highlight folded arginine side-chain amine groups (w1 = w1,app – 30 ppm). The “indole” peak is from Trp 144. The A and S are Ser and Ala residues at the N-and C-terminus.
