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Nucleotide binding to ClpB. Shown are the heat effects of titrating 50 μM wt ClpB (A) and ClpB(T213N/T612N) (B) with ATPγS (•) or ADP (○). Solid lines show fits of an empirical model of multiple ligand-binding sites (MCS ITC software, MicroCal Inc.). The enthalpy of binding is negative which indicates an exothermic reaction.
