Figure 4.

Interactions of ClpB with aggregated substrates. Wt ClpB, ClpB(T213N), ClpB(T612N), or ClpB(T213N/T612N) (1.5 μM protein) were incubated without or with aggregated 3 μM glucose-6-phosphate dehydrogenase (A) or 3 μM malate dehydrogenase (B) without nucleotides or with 5 mM ATP, ATPγS, or ADP. The solutions were passed through a 0.1-μm filter. SDS-PAGE analysis with a Coomassie stain is shown for the fractions retained on the filter and subsequently solubilized with an SDS buffer. Representative results from two independent experiments are shown.