Figure 5.

Reactivation of aggregated G6PDH in the presence of ClpB and the DnaK chaperone system. Aggregated 3 μM G6PDH was incubated without ClpB with 1 μM DnaK, 1 μM DnaJ, 0.5 μM GrpE (KJE) (+), KJE with 1.5 μM wt ClpB (•), KJE with 1.5 μM ClpB(T213N) (▵), KJE with 1.5 μM ClpB(T612N) (▴), or KJE with 1.5 μM ClpB(T213N/T612N) (♦). After incubation at 30°C for the indicated time, the G6PDH activity was measured as described in Materials and Methods. Representative results from two independent experiments are shown.