Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
(A) Nonenzymatic biotinylation of recombinant H2A using bio-5′-AMP as substrate. Panel (i), 0 time, 100 μM bio-5′-AMP; (ii) 60 min, 100 μM bio-5′-AMP; (iii) 180 min, 100 μM bio-5′-AMP, (iv) 420 min, 100 μM bio-5′-AMP. (B) Overnight incubation with 200 μM bio-5′-AMP. (C) Plot of ratio of unbiotinylated H2A:total H2A remaining versus time in enzymatic and nonenzymatic reactions. Ratios were estimated using peak heights of relevant species.
