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ELISA analysis and expression of Ser-substituted scFvs mutants. (A) ELISA of whole cell lysates incubated with 100 mM DTT and expressing: 
: 14B7* scFv, 
: 14B7* Cys4→Ser4 scFv, 
: 4-39 scFv, 
: 4-39 Cys4→Ser4 scFv. (B) Soluble expression level determined by Western blotting.
