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. Author manuscript; available in PMC: 2009 Jul 9.
Published in final edited form as: Virology. 2007 Nov 8;371(2):322–335. doi: 10.1016/j.virol.2007.10.010

Figure 2. Release of virus-like particles.

Figure 2

Cells were transfected with wt HIVgpt (wt HIV) or the indicated constructs and either untreated or treated with 1 μM PMA or PDB. At 72 h post-transfection, cell and VLP samples were collected, subjected to SDS-PAGE and anti-HIVCA immunoblotting. Cellular PrGag and VLP PrGag plus CA levels were quantitated densitometrically, raw release levels ([VLP PrGag + CA]/[Cell PrGag]) were calculated and are shown, normalized to untreated wt HIVgpt release levels. Results derive from one (wt HIV + PDB; PKCCBD-GFP-Gag + PDB), two (wt HIV + PMA; HIVgpt-MAGFP), three (PCKCBD-GFP-Gag + PMA), or four (GFP-AKTPH-Gag; GFP-PLCPH-Gag; PKCCBD-GFPGag) separate experiments. Standard deviations are as shown.