Figure 1. The mcm4^{Chaos3/Chaos3} mutant has a G2/M delay.

(A) The mutant shows a G2/M delay that is Rad9-dependent and Mad2-independent. (B) Homozygous mcm4^Chaos3 mutants have a higher mitotic index. Log phase cells were analyzed by microscopy. Cells with no bud (G1), small bud (S), and large bud (G2/M) were counted. (C) Microscopy of log phase mcm4^Chaos/Chaos3 and wild-type cells. (D) Fluorescence microscopy of DAPI-stained mutant cells. 77% of mutant large budded cells have one nucleus at the bud neck (pointed with white arrow), whereas 90% of large budded wild-type cells have two nuclei. (E) Serial dilutions of mcm4^Chaos3 homozgyotes and hemizygotes grown on YPD at 30°C and 37°C.