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. 2009 Jul 21;4(7):e6331. doi: 10.1371/journal.pone.0006331

Figure 2. PP6R proteins associate with the PP2A-like phosphatase catalytic subunit Sit4.

Figure 2

The quadruple sap mutant (JRY40) and sap185 sap190 double mutant (JRY29) strains were transformed with vector and derivatives expressing N-terminal FLAG tagged versions of PP6R1, PP6R2 and PP6R3. Proteins were immunoprecipitated with anti-FLAG antibody and analyzed by western blotting. The membrane was stripped and interaction with Sit4 was detected by reprobing with a Sit4 specific antiserum. Sit4 was also detected in equivalent amounts of protein extracts and Pgk1 served as a loading control.