Figure 5. PP6R3 partially enhances CLN1 expression and cell cycle progression.

Isogenic WT (MLY41) and quadruple sap (JRY40) mutant strains transformed with vector or plasmid expressing PP6R3 were grown to exponential phase and elutriated to enrich for G₁ cells. Elutriated cells were collected, transferred to fresh SD-Ura media, and samples were taken over a 3 hours period for G₁ functions analysis. (A) Samples were analyzed for DNA content by flow cytometry as in Figure 4A. The percentage of cells with 2N DNA content is shown in the upper right corner of each panel). (B) Table shows the percentage of budded cells (ND, not detectable). (C) CLN1 and ACT1 mRNA expression was assessed by northern blot. (D) Northern blot signals from experiment shown in Panel C for CLN1 were quantified and normalized to the ACT1 loading control signal. Results shown are the relative percentage of gene expression with maximal level of expression at 30 min for WT as 100%.