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. 2009 Jul 20;4(7):e6312. doi: 10.1371/journal.pone.0006312

Figure 3. Time curve and dose-dependency of thalidomide's effect on sprout formation of pig islets.

Figure 3

After cultivation of pig islets in medium containing 5 ng/ml bFGF and different concentration of thalidomide, the gels were fixed at the indicated times, stained and counted for percentage of islets with sprouts. A: Representative pictures of pig islet in the sprouting assay with or without sprouting activity (A1 and A2, respectively). B: The time curve of islet sprouting within 72 hours after cultivation. N = 3 in each time point. Vehvehicle (DMSO); THD 10thalidomide 10 µg/ml; THD100thalidomide 100 µg/ml. *p<0.05 vs Veh; **p<0.01 vs Veh.