FIG. 3.

Effects of the anti-GRP vaccine on the growth and angiogenesis of intradermal tumors. (A) Light microscope picture of B16-F10 tumor cells implanted intradermally in the anterior abdominal wall and the development of new blood vessels. Tumor-associated angiogenesis in mice injected with saline (a and b) and the pCR3.1-VS-HSP65-TP-M2 control vaccine (c and d) appeared to be significantly greater than in mice immunized with the pCR3.1-VS-HSP65-TP-GRP6-M2 anti-GRP vaccine (e and f). Representative images were taken with an objective that was ×10 (a, c, and e) or higher (b, d, and f). (B) The total number of blood vessels was determined within the precise 1-cm² area around each implant site. **, P < 0.01.