TABLE 2.
Performance of recombinant protein (pA104R, p54, pB602L, and pK205R)-based ELISAs compared to that of the conventional ELISA (OIE-ELISA)a
| Country (serum sample source) | Performance of the following assayb:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| OIE-ELISA
|
pA104R-ELISA
|
p54-ELISA
|
pK205R-ELISA
|
pB602L-ELISA
|
||||||
| Se | Sp | Se | Sp | Se | Sp | Se | Sp | Se | Sp | |
| Uganda (pigs) | 3/3 | 248/251 | 2/3 | 247/251 | 2/3 | 247/251 | 2/3 | 247/251 | 2/3 | 248/251 |
| Mozambique (pigs) | 4/4 | 85/91 | 1/4 | 88/91 | 3/4 | 90/91 | 3/4 | 83/91 | 3/4 | 90/91 |
| Nigeria (pigs) | 4/4 | 2/2 | 4/4 | 2/2 | 4/4 | 2/2 | 4/4 | 2/2 | 4/4 | 2/2 |
| Burkina Faso (pigs) | 5/5 | 15/15 | 5/5 | 15/15 | 5/5 | 15/15 | 5/5 | 15/15 | 5/5 | 15/15 |
| Uganda (warthogs) | 17/26 | 7/26 | 23/26 | 20/26 | 23/26 | |||||
a
A total of 375 serum samples obtained from domestic pigs in East Africa (Uganda, n = 254 [2 immunoblotting positive]; Mozambique, n = 95 [4 immunoblotting positive]) and West Africa (Nigeria, n = 6 [4 immunoblotting positive]; Burkina Faso, n = 20 [5 immunoblotting positive]) and 26 field serum samples obtained from warthogs in Uganda (all previously characterized as positive by immunoblotting) were analyzed.
b
Se, sensitivity; Sp, specificity. The data represent the number of serum samples positive (Se) or negative (Sp)/total number of serum samples tested.