TABLE 4.
Classification of OTUs clustering with free-living protozoa obtained from treated water and from biofilms in the distribution systems of water supply A and water supply Ba
| Organism(s) with highest similarity (GenBank accession no.)b | Similarity (%) | No. of OTUs | No. of clones
|
|||
|---|---|---|---|---|---|---|
| TW-A | BF-Ac | TW-B | BF-Bc | |||
| Amoebozoa | 10 | 3 | 4 | 2 | 8 | |
| Acanthamoeba polyphaga (AF260725) | 89.3 | 1 | 1 | |||
| Echinamoeba thermarum (AJ489264) | 85.7 | 1 | 1 | |||
| Eimeriidae environmental sample clone (EF024503)/Acanthamoeba sp. (AY173000) | 96.8/85.9 | 1 | 2 | |||
| Uncultured endolithic amoeba (AB257667)/Hartmannellidae sp. strain LO57N/1 (AY145442) | 79.2/77.9 | 1 | 7 | |||
| Neoparamoeba aestuarina (AY121851) | 89.3 | 1 | 1 | |||
| Pterocystis tropica (AY749612) | 93.4 | 1 | 1 | |||
| Raineriophrys erinaceoides (AY749633) | 94.7, 93.3 | 2 | 2 | |||
| Uncultured eukaryote clone (AY749523)/Raineriophrys sp. (AY749606) | 89.0/88.4 | 1 | 1 | |||
| Uncultured Sarcosomataceae clone (EF023269)/Amastigomonas mutalitis (AY050182) | 78.3/77.1 | 1 | 1 | |||
| Cercozoa | 39 | 14 | 60 | 24 | 4 | |
| Athalamea environmental sample clone (EF024169)/soil flagellate AND25 (AY965868) | 91.1/88.5 | 1 | 2 | |||
| Athalamea environmental sample clone (EF024169)/Hedriocystis reticulata (AY305010) | 90.3/85.0 | 1 | 1 | |||
| Athalamea environmental sample clone (EF024169)/Exuviaella pusilla (DQ388459) | 81.4/81.0 | 1 | 1 | |||
| Cercomonas longicauda (AY496047, AF411270, AY496047) | 96.3, 94.6, 91.9 | 3 | 4 | 1 | ||
| Cercomonas metabolicus (DQ211597) | 97.5, 95.6, 94.8 | 3 | 6 | |||
| Cercomonas sp. (AF534712) | 95.2, 95.0, 80.6 | 3 | 3 | 5 | ||
| Cercomonadida environmental sample clone (EF024293 and EF024163)/soil flagellate AND24 (AY965867) | 98.1, 93.4/94.1, 92.6 | 2 | 1 | 1 | ||
| Cercomonadidae environmental sample clone (EF024692)/Cercomonas sp. (AF411266) | 95.1/95.0 | 1 | 1 | |||
| Cercomonadida environmental sample clone (EF024163)/Masisteria marina strain DFS1 (AF174371) | 87.4/84.6 | 1 | 1 | |||
| Dimorpha-like sp. strain ATCC 50522 (AF411283) | 93.4 | 1 | 10 | |||
| Dodomorpha sp. strain HFCC57 (DQ211596) | 99.4 | 1 | 24 | |||
| Ebria triparrtita (DQ303922) | 88.2 | 1 | 3 | |||
| Pseudodiffllugia cf. gracilis (AJ418794) | 88.7 | 1 | 1 | |||
| Trachelocorythion pulchellum (AJ418789) | 76.5 | 1 | 13 | |||
| Uncultured Banisveld eukaryote clone (EU091827)/soil flagellate AND21 (AY965866) | 99.8/99.1 | 1 | 1 | |||
| Uncultured cercozoan sample clone (EF023523)/Cercomonadida environmental sample clone (EF024163) | 92.6/91.9 | 1 | 2 | |||
| Uncultured cercozoan clone (AY620301)/Cercomonas sp. (AF411266) | 98.0/91.4 | 1 | 1 | |||
| Uncultured cercozoan clone (AY620269)/Massisteria marina (AF174373) | 89.1/87.4 | 1 | 1 | |||
| Uncultured cercozoan clone (AY821946)/Pseudodiffllugia cf. gracilis (AJ418794) | 90.0/87.5 | 1 | 2 | |||
| Uncultured cercozoan partial 18S rRNA gene (AM114807)/soil flagellate AND24 (AY965867) | 99.0/93.0 | 1 | 3 | |||
| Uncultured cercozoan clone (AY620268)/Cercomonas sp. (AF411266) | 97.9/89.1 | 1 | 1 | |||
| Uncultured eukaryote clone (AY082981)/Ebria tripartita (DQ303922) | 96.1/88.0 | 1 | 1 | |||
| Uncultured eukaryote clone (EF024996)/Protaspis grandis (DQ303924) | 96.1/92.6 | 1 | 1 | |||
| Uncultured eukaryote clone (AY082993)/uncultured freshwater cercozoan (DQ243993) | 95.1/94.0 | 1 | 1 | |||
| Uncultured eukaryote clone (EF023764)/Protaspis grandis (DQ303924) | 99.4/94.0 | 1 | 1 | |||
| Uncultured freshwater cercozoan clone (DQ244000)/Cercomonadidae environmental sample clone (EF024294) | 83.3/81.4 | 1 | 1 | |||
| Uncultured freshwater cercozoan clone (DQ243992 and (DQ243993)/Cercomonas sp. (AF411271 and AF411266) | 92.6, 89.0/91.4, 88.5 | 2 | 2 | 1 | ||
| Uncultured freshwater cercozoan clone (DQ243993)/Rigidomastix-like sp. (AF411279) | 93.5/92.9 | 1 | 1 | |||
| Uncultured rhizosphere cercozoan (AJ506007)/soil flagellate AND21 (AY905866) | 78.9/74.5 | 1 | 1 | |||
| Unidentified eukaryote (AJ130856)/Lecythium sp. (AJ514867) | 97.0, 95.9/95.9, 94.4 | 2 | 3 | |||
| Choanozoa | 26 | 3 | 15 | 14 | 13 | |
| Amoebidium parasiticum strain ATCC 32708 (Y19155) | 90.5 | 1 | 1 | |||
| Codonosigidae evironmental sample clone (EF024012)/Monosiga ovata (AF271999) | 97.1/93.5 | 1 | 1 | |||
| Corallochytrium limacisporum (L42528) | 79.5 | 1 | 1 | |||
| Diaphanoeca grandis (DQ103820, AY753614, AF084234) | 92.9, 82.8, 75.7 | 3 | 3 | 3 | ||
| Eimeriidae environmental sample clone (EF024885)/Diaphanoeca grandis (AF084234) | 92.3/90.1 | 1 | 1 | |||
| Eimeriidae environmental sample clone (EF024885)/Endochytrium sp. (AY635844) | 91.9/90.2 | 1 | 1 | |||
| Eimeriidae environmental sample clone (EF023936)/Monosiga ovata (AF084230) | 94.9, 94.1, 90.2/92.1, 91.4, 88.9 | 3 | 2 | 1 | ||
| Eimeriidae environmental sample clone (EF024885)/Stephanoeca diplocostata (AF084235 and AY149899) | 91.5, 93.5/90.2, 92.8 | 2 | 7 | 1 | ||
| Ichthyophonus irregularis (AF232303 and AF232303) | 92.4, 79.4 | 2 | 2 | 1 | ||
| Nuclearia moebiusi (AF484686) | 91.8 | 1 | 1 | |||
| Rhinosporidium seeberi (AF118851) | 90.1 | 1 | 1 | |||
| Uncultured eukaryote (AB275066)/Diaphanoeca grandis (AF684234) | 90.7/89.6 | 1 | 1 | |||
| Uncultured eukaryotic picoplankton clone (AY642728)/Monosiga ovata (AF084230) | 94.8, 98.5, 90.7/94.4, 90.4 | 3 | 5 | 1 | ||
| Uncultured eukaryotic picoplankton clone (AY642707)/Stephanoeca diplocostata (AY149899) | 92.2/92.0 | 1 | 1 | |||
| Uncultured marine eukaryote clone (EF526879)/Corallochytrium limacisporum (L42528) | 90.4/89.9 | 1 | 2 | |||
| Uncultured marine eukaryote clone (EF526803 and DQ103820)/Diaphanoeca grandis (AF084234 and L10824) | 94.0, 88.7/92.4, 88.4 | 2 | 1 | 4 | ||
| Uncultured marine eukaryote clone (EF526803)/Stephanoeca diplocostata (AY149899) | 93.4/92.0 | 1 | 3 | |||
| Ciliophora | 29 | 7 | 4 | 17 | 21 | |
| Dexitrichides pangi (AY212805) | 91.2 | 1 | 2 | |||
| Heliophrya erhardi (AY007445) | 86.3, 85.6 | 2 | 5 | |||
| Hemiophrys macrostoma (AY102173) | 82.6 | 1 | 1 | |||
| Hemiophrys procera (AY102175) | 98.1, 96.8 | 2 | 2 | |||
| Holosticha diademata (DQ059583) | 98.7, 96.7 | 2 | 2 | |||
| Oxytrichidae environmental sample clone (EF024903)/Gonostomum namibiense (AY498655) | 97.5/96.9 | 1 | 1 | |||
| Parabirojimia similis (DQ503584) | 97.3 | 1 | 1 | |||
| Tokophrya lemnarum clone (AY332720) | 87.6 | 1 | 4 | |||
| Unidentified eukaryote (AJ130855)/Carchesium polypinum (AF401522) | 90.4/90.2 | 1 | 1 | |||
| Uncultured eukaryote clone (EF024996)/Dexitrichides pangi (AY212805) | 98.9, 87.1 | 1 | 1 | |||
| Unidentified eukaryote (AJ130851)/Ophrydium versatile (AF401526) | 85.9, 85.3 | 1 | 1 | |||
| Unidentified eukaryote (AJ130851)/Vorticella campanula (AF335518 and DQ662849) | 94.8, 92.4/94.6, 92.1 | 2 | 4 | |||
| Uncultured hypotrichid ciliate clone (AY821937)/Aspidisca steini (AF305625) | 88.9/87.7 | 1 | 2 | |||
| Uncultured marine eukaryote clone (DQ103847)/uncultured ciliate (AM114813) | 77.0/76.2 | 1 | 3 | |||
| Uncultured marine eukaryote clone (EF526916)/Plagiopyliella pacifica (AY541685) | 94.5/94.5 | 1 | 2 | |||
| Uroleptus gallina (AF164130) | 87.6 | 1 | 1 | |||
| Vorticella campanula (DQ662849 and AF335518) | 99.2, 98.4, 93.6, 95.2, 93.4, 91.6 | 6 | 1 | 7 | ||
| Vorticella fusca (DQ190468) | 99.3 | 1 | 1 | |||
| Vorticella sp. strain JCC-2006-4 (DQ868349) | 96.6 | 1 | 5 | 1 | ||
| Zoothamnium niveum (DQ868350) | 94.8 | 1 | 1 | |||
| Euglenozoa | 13 | 17 | 1 | 2 | 0 | |
| Bodo saltans (DQ207571); (AY490229) | 95.6, 77.2 | 2 | 2 | |||
| Neobodo designis (AY753616, AY753616, DQ207583) | 97.4, 94.3, 93.0, 91.1, 88.3, 87.7 | 6 | 10 | 1 | ||
| Petalomonas cantuscygni CCAP 1259/1 (AF386635) | 85.8 | 1 | 1 | |||
| Rhynchomonas nasuta (DQ207595 and AY425023) | 98.1; 85.4 | 2 | 4 | |||
| Uncultured eukaryote clone (AY753980)/Bodo saltans (AY490232) | 94.5/93.6 | 1 | 1 | |||
| Uncultured eukaryote clone (EF100316)/Petalomonas cantuscygni (U84731) | 80.5/78.5 | 1 | 1 | |||
| Myzozoa | 5 | 0 | 1 | 11 | 1 | |
| Pseudoperkinsus tapetis (AB300505) | 86.8 | 1 | 1 | |||
| Uncultured alveolate clone (AF372776)/Corallochytrium limacisporum (L42528) | 87.8/78.9 | 1 | 1 | |||
| Uncultured eukaryote clone (EF100258)/uncultured alveolate clone (AF372776) | 97.3/91.0 | 1 | 9 | |||
| Uncultured eukaryote clone (EF100258)/Colpodella pontica (AY078092) | 98.1/77.3 | 1 | 1 | |||
| Uncultured marine eukaryote clone (DQ103862)/Peridinium wierzejskii (AY443018) | 97.1/96.7 | 1 | 1 | |||
| Stramenopiles | 5 | 0 | 4 | 1 | 2 | |
| Aphanomyces invadans (DQ403202 and AF396684) | 98.7, 98.4 | 2 | 3 | |||
| Hyphochytrium catenoides (AF163294) | 97.3 | 1 | 2 | |||
| Paraphysomonas foraminifera (AB022864) | 98.5 | 1 | 1 | |||
| Rhizidiomyces apophysatus (AF163295) | 98.3 | 1 | 1 | |||
| Total | 127 | 44 | 89 | 71 | 49 | |
b
Most closely related sequence/most closely related genus or species. More than one accession number indicates that more than one OTU had the highest similarity to the same genus or species, but to different sequence types.
c
Data are totals for the three analyzed biofilm samples.