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. 2009 May 29;75(14):4770–4781. doi: 10.1128/AEM.00370-09

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FIG. 4. — PCR-RFLP analysis of the rRNA IGS region for differentiating the lineages of F. oxysporum f. sp. cubense. For this procedure, DNA obtained from a putative F. oxysporum f. sp. cubense isolate is used as a template for amplication of the IGS region (A), after which the amplicon is sequentially subjected to digestion with restriction enzymes AvaI (B), BceAI (C), BbvI (D), Csp6I (E), and BsrDI. (F) (B) Lane 1, 100-bp marker; lane 2, CAV 847 (lineage I); lane 3, CAV 613 (lineage II); lane 4, CAV 1100 (lineage III); lane 5, CAV 009 (lineage IV); lane 6, CAV 810 (lineage V); lane 7, CAV 957 (lineage VI); lane 8, CAV 608 (lineage VII); lane 8, CAV 189 (lineage VIII). (C) Lane 1, 100-bp marker; lane 2, CAV 794 (lineage I); lane 3, CAV 815 (lineage II); lane 4, CAV 294 (lineage III); lane 5, CAV 933 (lineage IV); lane 6, CAV 1098 (lineage V); lane 7, CAV 189 (lineage VI); lane 8, CAV (lineage VII); lane 8, CAV (lineage VIII). (D) Lane 1, 100-bp marker; lane 2, CAV 957 (lineage VI); lane 3, CAV 608 (lineage VII); lane 4, CAV 189 (lineage VIII). (E) Lane 1, 100-bp marker; lane 2, CAV 847 (lineage I); lane 3, CAV 613 (lineage II); lane 4, CAV 1100 (lineage III); lane 5, CAV 009 (lineage IV); lane 6, CAV 810 (lineage V). (F) Lane 1, 100-bp marker; lane 2, CAV 794 (lineage I); lane 3, CAV 815 (lineage II); lane 4, CAV 294 (lineage III); lane 5, CAV 933 (lineage IV).