FIG. 2.

Electrophoretic patterns on polyacrylamide gels and corresponding immunoblots of concentrated culture filtrates of P. oligandrum strains. (a and b) Coomassie blue-stained 12.5% SDS-PAGE gels. The same culture filtrates were subjected to 12.5% SDS-PAGE, and the proteins were transferred to nitrocellulose membranes. (c and d) The membranes were probed with rabbit anti-oligandrin serum. Each lane was loaded with 25 μg of proteins. Lanes M, molecular mass standard (Bio-Rad); other lanes, culture filtrates of the Pythium strains listed in Table 3.