TABLE 2.

Pairs of universal primers used in SSCP analyses of native rhizosphere fungal populations

Gene	Primer	Primer sequence (5′-3′)d	Amplicon size (bp)	Annealing temp (°C)
Mitochondrial large-subunit rDNA	ML1a	FAM-GTACTTTTGCATAATGGGTCAGC	253	58
	ML2a	FAM-TATGTTTCGTAGAAAACCAGC
ITS	ITS1a	TCCGTAGGTGAACCTGCGG	300	62
	ITS2a	FAM-GCTGCGTTCTTCATCGATGC
28S rDNA	U1b,c	FAM-GTGAAATTGTTGAAAGGGAA	260	56
	U2b,c	GACTCCTTGGTCCGTGTT

^aFrom White and coworkers (63).

^bFrom Sandhu and coworkers (50).

^cFrom Möhlenhoff and coworkers (32).

^dFAM, 6-carboxyfluorescein.