TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Characteristics | Reference or source |
|---|---|---|
| E. coli | ||
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqlacZΔM15 Tn 10 (Tetr)] | Stratagene |
| L. lactis | ||
| MG1363 | NCDO 712 derivative, plasmid- and phage-free strain | 13 |
| NZ9000 | MG1363 derivative, pepN::nisRK | 28 |
| Plasmid | ||
| pUC57 | Cloning vector, ColE1 origin, Ampr | Fermentas |
| pUC57-phaC | Codon-optimized phaC in EcoRV site of pUC57 | This study |
| pUC57-phaA | Codon-optimized phaA in EcoRV site of pUC57 | This study |
| pUC57-phaB | Codon-optimized phaB in EcoRV site of pUC57 | This study |
| pUC57-phaAB | Codon-optimized phaAB in pUC57 | This study |
| pUC57-ZZ | Codon-optimized gene for ZZ domain in EcoRV site of pUC57 | This study |
| pNZ8148 | Cmr, pSH71 origin, PnisA | 35 |
| pNZ-AB | pNZ8148 derivative, PnisA-phaAB | This study |
| pNZ-CAB | pNZ8148 derivative, PnisA-phaCAB | This study |
| pNZ-ZZB | pNZ8148 derivative, PnisA-ZZ-phaB | This study |
| pNZ-ZZCAB | pNZ8148 derivative, PnisA-ZZphaC-phaAB | This study |