TABLE 2.

Metabolites in supernatants from batch-grown E. faecalis V583 and the ldh mutants^a

Strain or genotype	Aeration	Final OD600	Final pH	Metabolite concn (mM)
				Glucose	Citrate	Lactate	Formate	Acetate	Ethanol	Acetoin
V583	−	1.7	4.5	25.5	2.2	60.1	6.5	16.5	3.2	0.3
	+	2.6	4.4	4.9	1.8	56.3	3.9	45.4	0.2	7.2
Δldh-1	−	2.7	5.5	4.8	0.7	16	28.2	7.1	39.2	23.9
	+	3.0	6.3	0.2	0.4	11.4	17.4	32	15.4	21.1
Δldh-2	−	1.7	4.6	25.6	2.2	59.9	6.5	16.6	3.3	0.5
	+	2.7	4.4	4.3	1.8	55.0	2.5	44	0.3	8.7
Δldh-1 Δldh-2	−	2.8	5.9	4.0	0.2	5.3	27.3	8.5	41.7	21.1
	+	2.7	6.3	0	0.2	3.5	20.8	33.1	11.4	24.6

^aValues are averages of results from three separate experiments. The composition of the growth medium was as follows: glucose, 61 mM; citrate, 2.20 mM; lactate, 0.3 mM; formate, 1.4 mM; acetate, 15.7 mM; ethanol, 0.1 mM; and acetoin, 0.1 mM. OD₆₀₀, optical density at 600 nm.