FIG. 6.

The heavy subunit of the Gal/GalNac lectin colocalizes with KDEL (ER marker), but not β-COP (Golgi marker), in cells expressing EhRabAQ84L. (A) Western blot analysis of trophozoite lysates from wild-type (WT) and mutant (Q84L) cells using anti-KDEL antibody. Consistent with its specificity in mammalian cells, the antibody prominently recognizes two proteins of 78 kDa and 94 kDa. (B) IF microscopy of wild-type (a to c) and mutant (e to g) cells by using anti-Hgl or anti-KDEL antibodies. (C) IF microscopy of wild-type (a to c) and mutant (e to g) cells using anti-Hgl or anti-β-COP antibodies. In both panels B and C, colocalized antigens are shown in yellow (c and g) and corresponding differential interference contrast microscopic images (d and h) are provided. Bars, 10 μm.