TABLE 1.
Generated Mycosphaerella graminicola G protein transformants and primers used in this study
| Gene or position | M. graminicola transformant | Type | Primer | Primer sequence |
|---|---|---|---|---|
| MgGpa1 | IPO323 ΔMgGpa1-14 | Disruption strain | MgGpa1-F3 | TTCAGCGAAGCCGAGAATATC |
| IPO323 ΔMgGpa1-15 | Disruption strain | MgGpa1-R2 | CACATGCGCAGGTTCTCTTG | |
| IPO323 E-MgGpa1-13 | Ectopic transformant | |||
| MgGpa2 | IPO323 ΔMgGpa2-2 | Disruption strain | MgGpa2-F1 | GGCGTACAGCTTGCGAT |
| IPO323 ΔMgGpa2-6 | Disruption strain | MgGpa2-R2 | CATCAAGTTATGCAAGTTCCGC | |
| IPO323 E-MgGpa2-10 | Ectopic transformant | |||
| MgGpa3 | IPO323 ΔMgGpa3-21 | Disruption strain | MgGpa3-F2 | AGCAGTGGGAACAATGAGGAG |
| IPO323 ΔMgGpa3-33 | Disruption strain | MgGpa3-R4 | TGAATACTGAGCTGGCCCAT | |
| IPO323 E-MgGpa3-2 | Ectopic transformant | |||
| MgGpb1 | IPO323 ΔMgGpb1-3 | Disruption strain | MgGpb1-F4 | ACCACGAACAAAGTACACGCC |
| IPO323 ΔMgGpb1-22 | Disruption strain | MgGpb1-R4 | AGTCGACCTGAGACGGAGAAAG | |
| IPO323 E-MgGpb1-1 | Ectopic transformant | |||
| Left border of transposon (Tn7L) | S | ATAATCCTTAAAAACTCCATTTCCACCCCT | ||
| Right border of transposon (Tn7R) | N | ACTTTATTGTCATAGTTTAGATCTATTTTG |