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. 2009 May 1;8(7):945–956. doi: 10.1128/EC.00326-08

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FIG. 5. — Time-lapse images of EGFP-ChsB localization in a germ tube. Hyphae of the EB-5 grown on an MMG plate for 8 h were observed by fluorescence microscopy. Panels A, C, E, G, I, K, M, O, and Q are DIC images. Panels B, D, F, H, J, L, N, P, and R are GFP images. (A and B) 0 min; (C and D) 3 min; (E and F) 6 min; (G and H) 9 min; (I and J) 12 min; (K and L) 15 min; (M and N) 30 min; (O and P) 45 min; (Q and R) 60 min. Solid arrows indicate a hyphal tip, arrowheads indicate a septation site, and open arrows indicate a branching site. Bars, 10 μm.

FIG. 5. — Time-lapse images of EGFP-ChsB localization in a germ tube. Hyphae of the EB-5 grown on an MMG plate for 8 h were observed by fluorescence microscopy. Panels A, C, E, G, I, K, M, O, and Q are DIC images. Panels B, D, F, H, J, L, N, P, and R are GFP images. (A and B) 0 min; (C and D) 3 min; (E and F) 6 min; (G and H) 9 min; (I and J) 12 min; (K and L) 15 min; (M and N) 30 min; (O and P) 45 min; (Q and R) 60 min. Solid arrows indicate a hyphal tip, arrowheads indicate a septation site, and open arrows indicate a branching site. Bars, 10 μm.