TABLE 2.
Straina | Production of ChsBb | Mean ± SD (%)c
|
|
---|---|---|---|
Colony diam (mm) | No. of conidia (106/mm2) | ||
B-1 | ChsB | 38.6 ± 1.0 (100) | 2.9 ± 0.3 (100) |
FB-3 | FLAG-ChsB | 36.6 ± 1.1 (95) | 2.8 ± 0.1 (97) |
EB-5 | EGFP-ChsB | 32.4 ± 1.4 (92) | 1.8 ± 0.1 (62) |
BM-3 | 5.8 ± 0.6 (15) | (>0.01) |
Strains were grown on the Y2G plates for 72 h.
It is likely that a very small amount of ChsB was also produced from alcA(p)-chsB in each strain by the leaky expression of alcA promoter under the condition used in this study (see the text).
The ratio of growth rate and conidiation efficiency of each strain to that of the wild-type strain is shown in parentheses. The conidiation efficiency of BM-3/A1 was below the measurable level.