TABLE 1.

Sequences of primers (F and R) and TaqMan probes (P) used for real-time PCR, target locations, and lengths of amplification products

Primer or probe	Sequence (5′ to 3′)	Locationa	Product size (bp)
HPV 16 E6-F	GCACCAAAAGAGAACTGCAATGTT	85-108	152
HPV 16 E6-R	AGTCATATACCTCACGTCGCAGTA	197-236
HPV 16 E6-P	GGACCCACAGGAGCGACCCAGAAAGTTA	112-139
HPV 16 E7-F	CAAGTGTGACTCTACGCTTCGG	738-759	81
HPV 16 E7-R	GTGGCCCATTAACAGGTCTTCCAA	796-818
HPV 16 E7-P	TGCGTACAAAGCACACACGTAGACATTCGT	763-792
HPV 18 E6-F	CTATAGAGGCCAGTGCCATTCG	503-524	79
HPV 18 E6-R	TTATACTTGTGTTTCTCTGCGTCG	558-581
HPV 18 E6-P	CAACCGAGCACGACAGGAACGACTCCA	530-556
HPV 18 E7-F	TAATCATCAACATTTACCAGCCCG	721-744	113
HPV 18 E7-R	CGTCTGCTGAGCTTTCTACTACTA	810-833
HPV 18 E7-P	CGAGCCGAACCACAACGTCACACAATGTT	745-774
HPV 31, E6-F	AAGACCGTTGTGTCCAGAAG	428-447	106
HPV 31, E6-R	GTCTTCTCCAACATGCTATGC	511-534
HPV 31, E6-P	CGTCCTGTCCACCTTCCTCCTATG	488-511
HPV 31, E7-F	TGTGTTAGATTTGCAACCTGAG	592-613	78
HPV 31, E7-R	ACATCCTCCTCATCTGAGCT	669-649
HPV 31, E7-P	CAACTGACCTCCACTGTTATGAGCAAT	615-641
HPV 33, E6-F	TGCACGACTATGTTTCAAGAC	100-120	132
HPV 33, E6-R	CTCAGATCGTTGCAAAGGTTT	211-231
HPV 33, E6-P	ATTCCACGCACTGTAGTTCAATGTTGT	179-205
HPV 33, E7-F	TTGTAACCTGTTGTCACACTTG	733-754	88
HPV 33, E7-R	AGTAGTTGCTGTATGGTTCGTA	799-820
HPV 33, E7-P	ACTTGCTGTACTGTTGACACATAAACGA	767-794

^aNucleotide positions based on genome sequences for HPV-16, -18, -31, and -33 (GenBank accession numbers K02718, X05015, J04353, and M12732, respectively).