TABLE 1.
Alleles of HAdV MicrosatI identified in GenBanka
| Accession no. | Serotype (species) | Microsatellite sequence | Allele (including extensions) |
|---|---|---|---|
| AY599834 | 3 (B1) | CCCttTTTTTTTTTTTTTTgTTTTTGTCGC | T(16)GT(5) |
| AY599836 | 3 (B1) | CCCttTTTTTTTTTTgTTTTTTGTTGA | T(12)GT(6) |
| AY594255 | 7 (B1) | GCCCtTTTTTTTTTTTgTTTTTGTCGC | T(12)GT(5) |
| AY601634 | 7 (B1) | CCCTCTTTTTTTTTTTgTTTTTTGTTGC | T(11)GT(7) |
| AY495969 | 7 (B1) | CCCTCTTTTTTTTTTTTgTTTTTTGTTGC | T(12)GT(6) |
| AY601636 | 16 (B1) | CCCTCTTTTTTTTgTTTTTTgTTGCCCA | T(8)GT(6)GT(2) |
| AY601633 | 21 (B1) | CCCTCTTTTTTTgTTTTTTTgTTGCCCA | T(7)GT(7)GT(2) |
| AF532578 | 11 (B2) | TCCTATTTTTTTTTTTTTGCCGC | T(13) |
| AY598970 | 11 (B2) | TCCTATTTTTTTTTTTTGCCGC | T(12) |
| AY803294 | 14b (B2) | TCCTATTTTTTTTTTTGCCGC | T(11) |
| AY737797 | 34 (B2) | TCCTATTTTTTTTTTTTGCCGC | T(12) |
| AY271307 | 35 (B2) | TCCTATTTTTTTTTTTTTTTTGCCGC | T(16) |
| AY128640 | 35 (B2) | TCCTATTTTTTTTTTTTTTTGCCGC | T(15) |
| AY737798 | 50 (B) | CCCTCTTTTTTTgTTTTTTTgTTGCCCA | T(7)GT(7)GT(2) |
| AY601635 | 5c (C) | GCCCCTTTTTTgcTTTTCCCAG | T(6)GCT(4) |
| DQ393829 | 49c (D) | CCCCCTTTTTTcTTTTTGCCAG | T(6)CT(5) |
| AY487947 | 4 (E) | CCCTCTTTTgTTTTgTTTTTTGCCAG | T(4)GT(4)GT(6) |
| AY599837 | 4 (E) | CCCTCTTTTgTTTTgTTTTTGCCAG | T(4)GT(4)GT(5) |
| L19443 | 40 (F) | GAGTCTTTTTgTTgTTTTTTGTAGA | T(5)GT(2)T(6) |
This table shows unique microsatellite alleles found in GenBank (BLASTN version 2.2.18; accessed 3 February 2008) for various strains of HAdV from BLASTN alignments with 5 bp of flanking sequence on each side identified using a 200-bp fragment surrounding an HAdV-14 T(17) allele as the query. Apparent base mutations that either shorten the microsatellite by interruption or elongate it by extension are shown in lowercase type. The 5′ flanking sequence of MicrosatI differs between genomic DNA and RNA transcript sequences, but the microsatellite itself does not. This suggests that the end of the microsatellite is a splice point. The expressed RNA, in which the microsatellite appears at the 3′ end, codes for the terminal binding protein. The position analyzed is bp 10780 in AY601634, from the complete genome of HAdV-7 (10).
From the prototype deWit strain of HAdV-14, isolated from The Netherlands recruits in the 1950s. Note that it is different from all alleles seen in the current outbreak of HAdV-14.
Many other species D serotypes share the same allele as serotype 49, and all four species C serotypes (HAdV-1, HAdV-2, HAdV-5, and HAdV-6) share the same allele. The microsatellite generally appears to be invariable in these two species.