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Construction of molecular proviral clones containing deletions and the EGFP reporter gene. ΔX indicates a deletion of gag-PR, PR, RT, or IN. The deletions were generated in the 5′-hemigenomic molecular clone p83-2 by inverse PCR and self-ligation (A), followed by subcloning into pNL4.3-EGFP (B). (C) Schematic representation of generated vectors. (Adapted from reference 53 with permission of the publisher.)
