TABLE 2.

Pyrosequencing signature sequences for C. parapsilosis and related species^a

Organism	Pyrosequencing profile	% Similarity	No. of strains sequenced
			Reference	Clinical
C. parapsilosis	GTCGAA-TTT GGAAGAAGTT TTGG-AGTTT GTACC	33	1	68
C. orthopsilosis	GTCGAA-TTT GGAAGAA-TT TTGG-AGTTT GTACC	32	10	1
C. metapsilosis	GTCGAA-TTT GGAAGAAtgT TTGG-AGTTT GTACC	31	2	1
L. elongisporus	GTCGAAgTTT GaAAtAtaga TTGG-AGcTT tTAtt	23		2
C. albicans	GTCaAAgTTT GaAgatAtac gTGGtAGacg tTACC	20		21

^aThe first 33 nucleotides of the C. parapsilosis sequence generated by Pyrosequencing analysis are shown; dashes (denoting the positions of indels) have been introduced to improve alignments. Nucleotides in uppercase are conserved; those in lowercase are positions that differ from the C. parapsilosis sequence. The number of nucleotides shared with the C. parapsilosis sequence (similarity) and the number of clinical and reference isolates tested which gave identical Pyrosequencing signature profiles (“Strains sequenced”) are shown. The signature sequences of C. albicans and Lodderomyces elongisporus (a teleomorphic yeast with physiological profiles similar to C. parapsilosis) are included for comparison.