TABLE 3.

NDV strains tested by the NDV-pathotyping RRT-PCR and their results in the specificity assay

NDV straina	Host	GenBank accession no.	Genotype	Virulenceb	RRT-PCR specific forc:
					Lentogens		Velogens/mesogens
					Test result	CT ± SD	Test result	CT ± SD
HK-99/75	Duck	EU604269	I	L	+	34.0 ± 1.1	−	NA
HK-138/80	Duck	EU604270	I	L	+	18.9 ± 0.8	−	NA
Ulster 2/C	Chicken	AY562991	I	L	+	23.6 ± 0.2	−	NA
PHY-LMV42	Chicken	DQ097394	I	L	+	20.7 ± 0.5	−	NA
Queensland V4	Chicken	AF217084	I	L	+	16.8 ± 0.3	−	NA
CA-12/63	Turkey	EU604268	II	L	+	14.7 ± 0.2	−	NA
BG-13/69	Chicken	EU604248	II	V	−	NA	+	25.8 ± 1.0
UK-Eaw/62	Chicken	EU604260	II	V	−	NA	+	18.4 ± 0.4
B1	Chicken	AF309418	II	L	+	16.4 ± 0.2	−	NA
LaSota	Chicken	AJ629062	II	L	+	15.5 ± 0.4	−	NA
HU/ck/146-V/95	Chicken	EU604271	II	L	+	20.8 ± 0.7	−	NA
Beaudette C	Chicken	X04719	II	M	−	NA	+	16.1 ± 0.3
KR-1/49	Chicken	EU604814	III	V	−	NA	+	16.9 ± 0.4
Mukteswar	Chicken	EF201805	III	M	−	NA	+	13.8 ± 0.3
H/Ph	Chicken	FJ687488	III	M	−	NA	+	14.5 ± 0.2
Herts'33/L	Chicken	FJ687487	H33 W	V	−	NA	+	21.5 ± 0.4
BG-60/81	Chicken	EU604266	IV	V	−	NA	+	20.7 ± 0.5
IT-7/60	Chicken	EU604256	IV	V	−	NA	+	15.7 ± 0.5
MA-307/77	Chicken	EU604259	IV	V	−	NA	+	21.3 ± 0.3
BG-25/78	Chicken	EU604249	V	V	−	NA	+	17.0 ± 0.3
HU-7/72	Chicken	EU604252	V	V	−	NA	+	13.3 ± 0.1
KR-2/84	Chicken	EU604258	V	V	−	NA	+	12.9 ± 0.5
PE-8/85	Chicken	EU604263	V	V	−	NA	+	15.5 ± 0.3
BG-29/86	Chicken	EU604267	VI	V	−	NA	+	15.2 ± 0.7
GR-12/68	Chicken	EU604251	VI	V	−	NA	+	13.2 ± 0.3
IQ-218/78	Pigeon	EU604254	VI	M	−	NA	+	13.6 ± 0.2
Israel 70	Chicken	EU604255	VI	V	−	NA	+	13.5 ± 0.3
KR-5/99	Chicken	EU665683	VI	V	−	NA	+	17.1 ± 0.1
CA-48/91	Pigeon	FJ687489	VI	M	−	NA	+	12.6 ± 0.3
HU-238/84	Pigeon	FJ687486	VI	M	−	NA	+	15.3 ± 0.2
IT-227/82	Pigeon	AJ880277	VI	M	−	NA	+	15.9 ± 0.4
BE-14/93	Chicken	EU604247	VII	V	−	NA	+	15.2 ± 0.2
BG-31/96	Chicken	EU604250	VII	V	−	NA	+	15.8 ± 0.1
DE-82/94	Chicken	EU604265	VII	V	−	NA	+	25.4 ± 0.2
ID-1/88	Cockatoo	EU604253	VII	V	−	NA	+	13.9 ± 0.2
TR-2/96	Chicken	EU604264	VII	V	−	NA	+	23.4 ± 0.3
IT-147/94	Turkey	EU604262	VIII	V	−	NA	+	28.8 ± 0.1
JP-382/84	Chicken	EU604257	VIII	V	−	NA	+	29.0 ± 0.1
ZA-16/90	Chicken	EU604261	VIII	V	−	NA	+	30.8 ± 0.3

^aFor APMV-1 isolates, the year/country of isolation are indicated in the designation of the virus (e.g., ZA-16/90: country of origin, South Africa; year of isolation, 1990; see also ISO 3166 two-letter country code standard for details [http://www.iso.org]).

^bL, lentogenic; M, mesogenic; V, velogenic.

^cNA, not applicable; +, postive; −, negative.