TABLE 5.
Results of lentogen-specific TaqMan MGB and gel-based PCR assays for organ and swab samples from experimentally infected chickens
| Samplea | Test result of PCR
|
||
|---|---|---|---|
| Lentogen-specific TaqMan MGB assayb
|
Gel-based assay using NDV primers A + Bc | ||
| Result | CT ± SD | ||
| Sampling at 2 days p.v. | |||
| Brain | − | NA | Negative |
| Conjunctiva | ++ | 27.8 ± 0.7 | Positive |
| Trachea | + | 33.5 ± 0.6 | Negative |
| Cecal tonsil | − | NA | Negative |
| Lung | + | 31.7 ± 0.2 | Negative |
| Oral swab | + | 30.3 ± 0.2 | Negative |
| Cloacal swab | − | NA | Negative |
| Sampling at 5 days p.v. | |||
| Brain | + | 38.0 ± 2.0 | Negative |
| Conjunctiva | ++ | 27.4 ± 0.5 | Positive |
| Trachea | ++ | 26.4 ± 0.4 | Positive |
| Cecal tonsil | − | NA | Negative |
| Lung | + | 31.7 ± 0.3 | Negative |
| Oral swab | + | 33.0 ± 0.4 | Negative |
| Cloacal swab | + | 31.7 ± 0.2 | Negative |
| Sampling at 9 days p.v. | |||
| Brain | − | NA | Negative |
| Conjunctiva | + | 35.9 ± 0.5 | Negative |
| Trachea | +/− | 42.9 ± 0.8 | Negative |
| Cecal tonsil | +/− | 43.1 ± 0.7 | Negative |
| Lung | − | NA | Negative |
| Oral swab | − | NA | Negative |
| Cloacal swab | ++ | 28.2 ± 0.2 | Positive |
a
Samples were taken 2, 5, and 9 days p.v. of chicks at 1 day of age with NDV strain PHY-LMV42 (Vitapest).
b
RRT-PCR test result: strong positive (++), CT < 30; positive (+), 30 < CT < 40; weak positive (+/−), CT > 40; negative (−), at least two negative results in triplicate reactions (CT is not applicable). NA, not applicable.
c
See reference 29.