TABLE 2.
Experimental results for repetitively quantified (20 times) serial dilutions of HIV-1 DNA standards in LightCycler 1.0 and LightCycler 2.0 platformsa
| Exptl platform | No. of HIV-1 DNA copies per reaction | No. of HIV-1 DNA copies/106 PBMCs | Mean ± SD of exptl valuesb | CV (%) of exptl valuesb |
|---|---|---|---|---|
| LightCycler 1.0 | 106 | 4 × 106 | 5.99 ± 0.08 | 1.36 |
| 105 | 4 × 105 | 5.08 ± 0.20 | 3.86 | |
| 104 | 4 × 104 | 3.95 ± 0.09 | 2.16 | |
| 103 | 4 × 103 | 2.99 ± 0.15 | 5.02 | |
| 102 | 400 | 2.21 ± 0.27 | 12.37 | |
| 25 | 100 | 1.44 ± 0.27 | 18.87 | |
| 12.5 | 50 | 0.79 ± 0.21 | 25.93 | |
| LightCycler 2.0 | 10 | 40 | 1.03 ± 0.13 | 12.99 |
| 6.25 | 25 | 0.87 ± 0.31 | 35.32 | |
| 3.125 | 12.5 | 0.17 ± 0.40 | 232.26 |
a
Each reaction was performed in a multiplex setting with 5 × 105 copies of the CCR5 gene in a final reaction volume of 25 μl. The experimental results were 100% positive for all concentrations with both platforms.
b
Log10-transformed values.