TABLE 3.

Quantified values for serial dilutions of standards with LightCycler 2.0 platform^a

Target DNA	No. of copies of DNA per reaction	No. (%) of measurements above the limit of detection for 28 replicates (HIV-1) or 12 replicates (CCR5)	Mean ± SD of exptl valuesb	CV (%) of exptl valuesb
HIV-1	106	28 (100)	5.89 ± 0.12	2.0
	105	28 (100)	4.93 ± 0.16	3.2
	104	28 (100)	3.83 ± 0.16	4.0
	103	28 (100)	2.97 ± 0.12	4.0
	102	28 (100)	2.03 ± 0.24	11.6
	10	9 (32.1)	1.00 ± 0.27	27.3
CCR5	106	12 (100)	5.92 ± 0.29	4.9
	105	12 (100)	5.03 ± 0.21	4.2
	104	12 (100)	3,94 ± 0.12	2.9
	103	12 (100)	3.06 ± 0.20	6.6
	102	12 (100)	2.05 ± 0.19	9.3
	10	12 (100)	1.05 ± 0.29	27.6

^aEach reaction was performed in a multiplex setting, with each reaction mix including, in addition to the standards, 10³ copies of HIV-1 DNA and 5 × 10⁵ CCR5 copies for CCR5 and HIV-1 DNA quantifications, respectively, in a final volume of 25 μl.

^bLog₁₀-transformed data.