FIG. 3.
Quantification of proinflammatory gene transcripts in the spleens of Igh6−/− and C57BL/6 mice infected with B. abortus 2308 or the virB mutant. Transcript levels in spleens from groups of four mice infected with either B. abortus 2308 (black bars) or the virB mutant (white bars) were assayed by real-time reverse transcriptase PCR at different time points postinoculation. Increases in transcript levels were calculated by normalizing CT values for each of the gene levels to CT values for GAPDH levels and to CT values for each of the gene levels measured in a group of mock-infected C57BL/6 mice. Asterisks indicate significant differences (α = 0.05) between Igh6−/− and control mice as calculated by analysis of variance.