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. 2009 May 4;77(7):3109–3116. doi: 10.1128/IAI.01460-08

FIG. 6.

FIG. 6.

Expression of E-selectin in HUVECs after stimulation with H. pylori bacteria. The expression of E-selectin was evaluated on HUVECs by flow cytometry after 6 h of stimulation with a 50-μl H. pylori suspension (OD of 1.0), 100 ng/ml of TNF-α, or PBS (unstimulated control). (A) E-selectin (solid lines) and isotype control (dashed lines) expression levels on HUVECs incubated with PBS (negative control), TNF-α (positive control), and H. pylori wt strains P1 and P12. (B) E-selectin (solid line) and isotype control (dashed line) expression levels on HUVECs after stimulation with H. pylori isogenic mutants P1ΔvacA, P1ΔcagA, P1ΔnapA, P1ΔureA, P1ΔvirD4, and P1ΔvirB10. The results shown are from one representative experiment out of three to six experiments.