FIG. 3.

Proton NMR spectrum of legiobactin. Purified legiobactin in deuterium oxide was subjected to 300-MHz proton NMR. The protons of legiobactin were detected from 1.1 to 4.2 ppm. A large DOH peak was present at 4.8 ppm due to the deuterium picking up a hydrogen atom, and water suppression experiments showed that no legiobactin-related peaks were hidden under the D₂O peak. The peak near 10 ppm was not observed in repeat experiments. The results presented are representative of three independent experiments.