FIG. 5.

HPLC analysis of CDM culture supernatants from lbtA and lbtB mutants. Concentrated supernatants obtained from deferrated CDM cultures of wild-type 130b, lbtA mutant NU300, and complemented mutant NU300(plbtA) (A) as well as lbtB mutant NU303 and complemented mutant NU303(plbtB) (B) were injected onto a TSK-GEL DEAE-2SW anion-exchange column and then subjected to NaCl elution over a 120-min period. Shown here are those peaks eluted between 80 and 90 min, as detected at A₂₂₀ (top line) and A₂₅₄ (bottom line). The results presented are representative of at least three independent experiments. AU, arbitrary units.