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Distribution of Stx1 and Stx2 in EHEC. Stx1-producing EHEC K24, Stx1- and Stx2-producing EHEC EDL933 (EDL), and Stx2-producing EHEC 86-24 were cultured in LB broth for 12 h at 37°C and fractionated into supernatant (S), periplasmic (Per), and cytoplasmic (C) fractions. The samples were analyzed by SDS-PAGE and immunoblotting using anti-Stx1 antiserum or anti-Stx2A MAb VT2-32. The anti-GroEL and anti-RNA polymerase α subunit antibodies were used as experimental controls.
