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Silver-stained SDS-PAGE of LPS preparations from strains Nissle 1917 (EcN) and EcNK5−. LPS was purified from strains Nissle 1917 and EcNK5− and analyzed by SDS-PAGE, followed by silver staining. The LPS profiles of strains Nissle 1917 and EcNK5− are identical, indicating that the kfiC mutation in strain EcNK5− has no detectable effect on the structure of the LPS.
