FIG. 7.

Furin-deficient cells can efficiently proteolyse PE, but they poorly process the LRP. (A) PE processing. Where indicated, PE (40 nM) was added to CHO (wild type) or CHO FD11 (furin-deficient) cells for 4 h before immunoprecipitation and anti-PE Western blotting. The molecular masses of markers (in kDa) are indicated on the left. The positions of the 55-kDa and 37-kDa fragments are indicated by an arrowhead and an arrow, respectively. (B) LRP processing. LRP was immunoprecipitated before anti-LRP Western blotting. The positions of the precursor (600 kDa) and the large subunit of the mature LRP (515 kDa) are indicated on the right. Lysate blots were probed for actin as a loading control.