FIG. 4.

Effect of l-proline on migration of T. cruzi metacyclic forms through gastric mucin. Parasites previously maintained for 36 h in PBS were added to the bottom of 24-well plates, in the absence or presence of 1 mM glucose or 3 mM l-proline. After 1 h of incubation at 37°C, polycarbonate Transwell filters coated with gastric mucin were placed onto parasite-containing wells. At different time points, samples from the filter chamber were collected and the numbers of parasites counted. Values represent the means ± standard deviations of the results of three independent experiments performed in duplicate.