TABLE 1.
Effect of rotenone plus antimycin A on T. cruzi ATP content and infectivitya
| T. cruzi strain | Concn (μM)
|
ATPb (%) | HeLa cell invasionb (%) | ||
|---|---|---|---|---|---|
| Antimycin A | Rotenone | l-proline | |||
| CL | 0 | 0 | 0 | 100.0 | 100.00 |
| 0.5 | 1 | 0 | 35.3 + 3.9 | 41.3 + 0.8 | |
| 0.5 | 1 | 3.0 | 57.8 + 2.4 | 68.2 + 5.9 | |
| 0.5 | 10 | 0 | 30.8 + 2.3 | 34.0 + 5.6 | |
| 0.5 | 10 | 3.0 | 49.2 + 6.4 | 67.7 + 7.3 | |
| 0.5 | 50 | 0 | 32.2 + 3.7 | 28.8 + 2.5 | |
| 0.5 | 50 | 3.0 | 59.0 + 2.5 | 66.5 + 8.4 | |
| G | 0 | 0 | 0 | 100.0 | 100.00 |
| 0.5 | 1 | 0 | 135.7 + 11.7 | 120.94 + 5.6 | |
| 0.5 | 1 | 3.0 | 225.8 + 4.4 | 89.8 + 4.9 | |
| 0.5 | 10 | 0 | 44.9 + 2.1 | 45.9 + 2.8 | |
| 0.5 | 10 | 3.0 | 79.0 + 2.0 | 50.8 + 3.7 | |
| 0.5 | 50 | 0 | 23.3 + 1.6 | 32.3 + 2.1 | |
| 0.5 | 50 | 0.75 | 45.1 + 0.6 | 36.5 + 3.2 | |
a
Parasites were incubated with antimycin A plus rotenone at the indicated concentrations for 30 min. After washings in PBS, l-proline was added and incubation proceeded for 1 h before ATP measurement and determination of infectivity.
b
Values represent means + standard deviations of the results of triplicate experiments.