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. 2009 Apr 13;77(7):2866–2875. doi: 10.1128/IAI.00039-09

FIG. 1.

FIG. 1.

Western blot analysis of bacterial culture supernatants (A) and pellet fractions (B) of wild-type Sal 18 and the mutant strains constructed, using rabbit anti-His tag SipD polyclonal serum. Lane 1, prestained protein marker; lane 2, wild-type Sal 18; lane 3, Sal 18 ΔSPI-1; lane 4, Sal 18 ΔinvG; lane 5, purified His tag SipD (38.5 kDa). The SipD protein (37 kDa) was present in the supernatant of the wild-type strain (A, lane 2) but not in the Sal 18 ΔSPI-1 and Sal 18 ΔinvG strains (A, lanes 3 and 4, respectively). In the pellet fractions, SipD was present (*) in the wild-type strain pellet (B, lane 2), but not in the Sal 18 ΔSPI-1 pellet (B, lane 3). The Sal 18 ΔinvG pellet contained some SipD, but not as much as the wild type (B, lane 4).