FIG. 2.

Expression of ers and ace determined by Western blot or RT-qPCR analysis. (A) Western blot analysis (with antiserum against His-tagged Ers) of E. faecalis protein extracts (40 μg) from strain JH2-2 grown at 37°C (lane 2), at 46°C (lane 3), or in the presence of bile salts (lane 4) and from strain JH2-2/p3535ers (lane 5). A 0.1-μg sample of purified His₆-Ers protein was loaded into lane 1. (B) RT-qPCRs experiments with the ers and ace genes and primers described in Table 2. RNAs were extracted from cells cultured under the same conditions used for protein extracts. Values that are significantly different are >2 (P < 0.05).