TABLE 2.
Primers used in this study
| Locusa or primer name | Sequence (5′-3′)a
|
Use | |
|---|---|---|---|
| Forward | Reverse | ||
| ers | CGCGGATCCATAATAGATAATTGAGG | TTGCTGCATCTTTTTCATGG | RT-qPCR |
| ef0082 | TTGACGTCAGCACCTTCTTC | CGTAGCGTTCACCTTTGACA | RT-qPCR |
| ef0049 | CAGCTTTTCCGACACGTCTT | TTCCCCCACGTACTTTAGCA | RT-qPCR |
| ef0782 | TTAGAGCGCGCAGTGAATC | ATGGCCGATCTGCTTCTAAA | RT-qPCR |
| ace | ATGAAGGAAGCCCACAGTTG | GTTGTGCCTGTTTTGGGAAG | RT-qPCR |
| kat | GCTGTTTGGGATTTTTGGTC | CATGCATATGACGGAACGAC | RT-qPCR |
| ef1656 | TTTATTTGTCACCCAACCAACA | CAATTGAAACGGTTGATCAGAA | RT-qPCR |
| ef1664 | TGGCGACCTAATTACCTTGG | CGTTTGCATCGTACGTGAGT | RT-qPCR |
| ef1816 | CGTCAAGACGATACCCGATT | TTTCCATGTTGTGCAGGAAG | RT-qPCR |
| ef2185 | GCGATTGCTCCAACTGAATC | GCCGTCCGTAATAAAAAGCA | RT-qPCR |
| ef3146 | CCAGAATTCTCAATTCCTCGTT | CAAATTTTTGTACACCGACAGG | RT-qPCR |
| aceRace1 | ATTCCAACTAGTGCGTCTGG | 5′RACE PCR | |
| aceRace2 | TTGAGGTCATCTCTCCTTG | 5′RACE PCR | |
| aceRace3 | TCAATTCGGCGCCAAACGAA | 5′RACE PCR | |
| Prom-ace | TCATTCTTTTCCTCCTTATCT | CCGCAATTGGTAGAATCATTA | Cloning in pGEM-T |
| Prom-ers | TAAGTTTGGTTCTGTCATTA | CGACTCGAAAGGAATGTTCA | Cloning in pGEM-T |
| aceDC-Ub | CCGGAATTCAGCTCAACTATGCCTGTCGA | CGCGGATCCGTCATTCCAACTAGTGCGTC | Cloning in pMAD |
| aceDC-Db | CGCGGATCCCAGCCATCCACAGAAACAAC | GAAGATCTTTCCTCTGCCATTAACGCGT | Cloning in pMAD |
| p3535ers | CGCGGATCCATAATAGATAATTGAGGAGTTA | CGCGCGCTGCAGTTAAACAATAATGTTATCTCTAATC | Cloning in pMSP35535 |
| pQE30-ersHTH | GCGGATCCAAAAAGATGCAGCAAATGTTGAT | CGCGCGCTGCAGTTAAACAATAATGTTATCTCTAATC | Cloning in pQE30 |
| mad1F | TCTAGCTAATGTTACGTTACAC | Cloning verification | |
| mad2R | TCATAATGGGGAAGGCCATC | Cloning verification | |
| Pu | GTTGTAAAACGACGGCCAGT | Cloning verification | |
| Pr | CACAGGAAACAGATATGACC | Cloning verification | |
a
From the annotated sequence available at http://www.tigr.org.
b
Primers used to clone upstream (U) and downstream (D) sequences of ace in order to construct a deletion mutant and complemented strain by a double-crossover (DC) event.