TABLE 3.
Identification and expression of genes harboring a putative Ers box in the promoter region
| ORFd | Gene name | Putative Ers box | Expressiona (P value) with reference to that of:
|
|
|---|---|---|---|---|
| 23S rRNA gene | gyrA | |||
| ef0049 | AACAAATGTTC-N75-ATG | NDb | ND | |
| ef0782 | AACAAATGTTG-N272-ATG | +1.01 | +1.1 | |
| ef1099 | ace | AACAAATGTTA-N176-ATG | +5.11 (0.02) | +7.4 (0.016) |
| ef1656 | AACATATGTTA-N77-ATG | −1.17 | −1.72 | |
| ef1664 | AACAATTGTTA-N132-ATG | +1.12 | −1.01 | |
| ef1816 | AACAAATGTTT-N81-ATG | +1.36 | +1.27 | |
| ef2185 | AACATATGTTT-N129-ATG | —c | — | |
| ef3146 | AACAATTGTTT-N424-ATG | +1.99 | +2.4 (>0.05) | |
a
Shown is the level of activation or repression in the Δers mutant compared to the wild type determined by RT-qPCR. Significant values are in bold (value > 2 and P < 0.05).
b
ND, no detectable expression.
c
—, Absence of the locus in the JH2-2 genome.
d
ORF, open reading frame.