FIG. 5.
RevA-fibronectin interactions. (A) Binding of RevAB31 to immobilized fibronectin fragments, as analyzed by ELISA. Bound RevA was detected by using purified specific antibody. Data represent the means and standard errors of the results from two separate experiments with six replicates. The asterisks indicate values significantly different from those with binding of RevA to whole fibronectin (P < 0.05, Student's t test assuming unequal variances). Fn, full-length fibronectin; 70kDa, amino-terminal 70-kDa fragment of fibronectin; 30kDa, 30-kDa amino-terminal domain of the 70-kDa fragment; 45kDa, 45-kDa gelatin-binding domain of the 70-kDa fragment. (B) Role of ionic interactions in RevAB31 binding of fibronectin. Binding of fibronectin to immobilized RevA in the presence of increasing concentrations of NaCl was analyzed by ELISA. Data represent the means and standard errors of the results from three experiments with six replicates per concentration of NaCl. Fn, fibronectin; NS, not statistically significant. (C) Effects of heparin on RevAB31-fibronectin interactions. Data represent the means and standard errors of the results from three experiments with six replicates per concentration of heparin. Fn, fibronectin; NS, not statistically significant.