TABLE 1.

Oligonucleotide primer sequences used in this study

Name	Sequence (5′ to 3′)	Purpose
B. burgdorferi B31 N40 revA forward	TGTAAAGCATATGTAGAAGAAAAG	Cloning
B. burgdorferi B31 revA reverse	TTAATTAGTGCCCTCTTCGAGGAA	Cloning
B. burgdorferi N40 revA reverse	TTAATTGGTGCCCTCTTCGAGGAA	Cloning
B. burgdorferi 297 cp32-4 revA forward	TGTAAAGCATATGTAGAAGAAAAG	Cloning
B. burgdorferi 297 cp32-4 revA reverse	TTAATTAGTACCTTCTTCGAGAAA	Cloning
B. burgdorferi 297 cp32-12 revA forward	TGTAAAGCATATGTGGAAGAGAAG	Cloning
B. burgdorferi 297 cp32-12 revA reverse	TTAATTAGTACCCTCTTCAAGAAAC	Cloning
B. garinii PBi revA forward	TGTAAAACATATGTAAAAGAAAAAGAAGAG	Cloning
B. garinii PBi revA reverse	TCAATTAGTACCTTCTTCGAGAAACTTTA	Cloning
B. burgdorferi B31 revB forward	GAACTATTTATAATAAAAAGGAG	Cloning
B. burgdorferi B31 revB reverse	TTAATCTTCTTCAAGATATTTTATTAT	Cloning
B. burgdorferi B31 revAC1 F	ATGTCTAAAAACTAAAAGGGCGAGCTCAACGATC	Overlap PCR
B. burgdorferi B31 revAC1 R	CGCCCTTTTAGTTTTTAGACATTTTATCCCCATTACC	Overlap PCR
B. burgdorferi B31 revAC2 F	GACAAGGATCAGGATATTTCAAAAAGATAAAAGGGC	Overlap PCR
B. burgdorferi B31 revAC2 R	ATCGTTGAGCTCGCCCTTTTATCTTTTTGAAAT	Overlap PCR
B. burgdorferi B31 revAN1 F	GATGACGATAAGAATCATCCCTTCACCGATGTTTTA	Overlap PCR
B. burgdorferi B31 revAN1 R	AGAAGAATCATTTACAAGAGCTAAAACATCGGTGAAGGGATG	Overlap PCR
B. burgdorferi revA1	GAA ATA GAT TCA TTA ATG GAG GA	qRT-PCR
B. burgdorferi revA2	CTT GTC AGT AAT GTT TTC AAT GG	qRT-PCR
B. burgdorferi flaB3	GGG TCT CAA GCG TCT TGG	qRT-PCR
B. burgdorferi flaB4	GAA CCG GTG CAG CCT GAG	qRT-PCR