FIG. 6.

NOD1 modulates IL-6 and IL-1β responses after incubation with L. monocytogenes but not after incubation with heat-killed bacterial lysates or with bacteria unable to escape from the phagosome into the cytosol. Total RNA from BMM was extracted before and 6 h after infection with L. monocytogenes at a multiplicity of infection of 0.2 (A to D) or with Δhly L. monocytogenes at a multiplicity of infection of 10 (F) or after incubation with HKL (E). The titers of IL-1β, IL-6, IFN-β, CXCL1 (A to F) and HPRT mRNA were determined by real-time RT-PCR. The mean increases in cytokine or HPRT levels for triplicate (A to D) or duplicate (E and F) wells are shown. Two independent experiments were performed, and the results of one of them are shown.