FIG. 2.

FAP induces the expression of costimulatory and MHC class molecules on DCs in a dose-dependent manner. DCs were cultured for 24 h in the presence of 100 or 200 ng/ml FAP and analyzed by two-color flow cytometry. The cells were gated to exclude CD11c⁺ cells. Medium, untreated control; LPS, positive control (200 ng/ml LPS). DCs were stained with anti-CD80, anti-CD86, anti-CD40, anti-MHC class I, or anti-MHC class II. The results are representative of the results of three experiments.